Table 2. Characterization of genic SSR markers in L. usitatissimum and their transferability to 11 Linum species. |
Bank accession n° |
Locus name |
Primer sequence (5’→3’) |
Repeat motif |
Expected size bp |
N° alleles |
PIC |
Transferability to Linum species |
||||||||||||
L. b. |
L. p. |
L.a. |
L. g. |
L. n. |
L.l. |
L. h. |
L.t. |
L.s. |
L.ta. |
L. f. |
N° putative alleles |
Multi loci* |
|||||||
EX720235.1 |
LM-42 |
GAATGAGGAACACCGGAAAG |
(CCA)5 |
163 |
2 |
0.28 |
+ |
+ |
+ |
+ |
+ |
+ |
- |
+ |
+ |
- |
+ |
4 |
- |
EX720209.1 |
LM-43 |
GCTGACGAAGGAGAAGGAAG |
(GGA)5 |
172 |
3 |
0.55 |
+ |
+ |
+ |
+ |
+ |
- |
+ |
+ |
+ |
- |
+ |
4 |
- |
EX720185.1 |
LM-44 |
AACCAACAACTACGCCTTGG |
(ATG)6 |
171 |
1 |
M |
+ |
+ |
+ |
- |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
2 |
- |
EX720157.1 |
LM-45 |
GTGATGATGATGGTGGCG |
(TCT)5 |
209 |
3 |
0.54 |
+ |
+ |
+ |
- |
+ |
+ |
- |
- |
+ |
+ |
+ |
3 |
- |
EX720130.1 |
LM-46 |
CGGATCCTTATAGCCTGCTG |
(AAG)6 |
162 |
3 |
0.43 |
+ |
+ |
- |
+ |
- |
- |
- |
- |
- |
- |
- |
1 |
- |
EX720114.1 |
LM-47 |
CCGTTCACTTTCTCCTCTGC |
(AAG)5 |
161 |
1 |
M |
+ |
+ |
- |
- |
- |
+ |
- |
+ |
- |
- |
+ |
3 |
- |
EX720099.1 |
LM-48 |
GGAGGAATAGCGAGCACAAG |
(GCA)5 |
162 |
2 |
0.47 |
+ |
+ |
+ |
+ |
- |
- |
+ |
- |
- |
+ |
+ |
2 |
- |
EX720068.1 |
LM-49 |
GATCCCCAATCCGATCTTTC |
(AGG)5 |
208 |
2 |
0.40 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
- |
+ |
+ |
2 |
- |
EX720059.1 |
LM-50 |
CATCTTTGCAGTGAAGGTGG |
(GAT)5 |
188 |
2 |
0.29 |
- |
+ |
- |
+ |
- |
- |
+ |
- |
- |
- |
- |
1 |
- |
EX720044.1 |
LM-51 |
CGTCTGTTTCACTCCTCAGC |
(TCT)6 |
198 |
2 |
0.49 |
+ |
+ |
- |
+ |
- |
+ |
- |
- |
+ |
- |
+ |
2 |
- |
EX720477.1 |
LM-53 |
AGAGGAAAATGGAAGAGGCG |
(ACG)5 |
150 |
2 |
0.39 |
+ |
+ |
- |
+ |
- |
+ |
+ |
+ |
+ |
- |
+ |
2 |
- |
EX720432.1 |
LM-53 |
GCATGAGCTCAACAGTCCAG |
(CAG)5 |
203 |
1 |
M |
+ |
- |
- |
- |
+ |
- |
- |
- |
- |
- |
- |
1 |
- |
EX720405.1 |
LM-54 |
GGAACCGCAGCTGGACTTAG |
(AGA)6 |
189 |
2 |
0.50 |
+ |
+ |
- |
+ |
- |
+ |
+ |
- |
+ |
+ |
+ |
2 |
+ |
EX720361.1 |
LM-55 |
TAGACGAGACCCAGCAGAGC |
(AGA)5 |
154 |
4 |
0.39 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
2 |
+ |
EH792583.1 |
LM-56 |
AGCTTGTGATGCTGATGGTG |
(TAA)6 |
161 |
1 |
M |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
EH792520.1 |
LM-58 |
TCTCCTGCAGCCTCCACTAC |
(GCC)5 |
196 |
1 |
M |
+ |
+ |
- |
- |
- |
+ |
+ |
- |
+ |
- |
+ |
1 |
- |
EH792442.1 |
LM-59 |
ATTGGATGCTGGATGGAGAC |
(GCA)6 |
193 |
1 |
M |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
- |
+ |
+ |
+ |
2 |
+ |
EH792413.1 |
LM-60 |
AAGACGCTGCTGAATCATTG |
(GAA)5 |
162 |
2 |
0.49 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
- |
+ |
- |
+ |
1 |
- |
EH792342.1 |
LM-61 |
TGGGTGAAGAAAAGAAAGAGG |
(AAG)5 |
178 |
1 |
M |
- |
- |
- |
+ |
- |
- |
- |
- |
- |
- |
- |
1 |
- |
EH792318.1 |
LM-62 |
AACCAGCAGCTTCCAAAGAC |
(AGG)5 |
155 |
2 |
0.39 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
3 |
- |
EH792278.1 |
LM-63 |
TATTTCATGCACCGCAAAAC |
(GAG)6 |
180 |
1 |
M |
+ |
+ |
+ |
- |
+ |
+ |
+ |
+ |
+ |
- |
+ |
2 |
+ |
EH792257.1 |
LM-65 |
TTCACAAGGCCTAACCCATC |
(ATC)5 |
166 |
1 |
M |
+ |
+ |
+ |
+ |
- |
+ |
+ |
- |
+ |
- |
+ |
2 |
+ |
EH792225.1 |
LM-66 |
TTATTATTAATTCAACCACAACGC |
(TAT)7 |
152 |
1 |
M |
+ |
+ |
- |
- |
- |
- |
- |
- |
+ |
- |
- |
1 |
- |
EH792154.1 |
LM-67 |
ACTGCGAAATCGAGATCAGG |
(CCA)6 |
177 |
1 |
M |
+ |
+ |
- |
+ |
- |
+ |
+ |
- |
+ |
- |
- |
2 |
+ |
EH792070.1 |
LM-68 |
GAATTCCCGGGATATCGAAC |
(CAT)4(CAT)5 |
168 |
1 |
M |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
n/e |
EH792050.1 |
LM-69 |
ATACTTGGCTAGTGGTGGCG |
(TGA)5 |
155 |
2 |
0.44 |
+ |
+ |
- |
+ |
- |
- |
- |
+ |
+ |
- |
- |
1 |
- |
EH791974.1 |
LM-70 |
AAAGGCAGGCACATCAAGAG |
(GAA)6 |
205 |
3 |
0.41 |
+ |
+ |
+ |
+ |
- |
+ |
+ |
+ |
+ |
+ |
+ |
3 |
+ |
EH791902.1 |
LM-71 |
ATGACAATGCTCCTCCATCC |
(CAT)5 |
168 |
1 |
M |
+ |
+ |
- |
+ |
- |
- |
- |
- |
- |
- |
- |
1 |
- |
EH791837.1 |
LM-72 |
CTTCCCCTTCTCCTCCTCAG |
(GGC)6 |
168 |
2 |
0.30 |
+ |
+ |
- |
+ |
- |
+ |
- |
- |
+ |
- |
- |
1 |
- |
EH791736.1 |
LM-73 |
ATACCCTACTGTTGCTGGGC |
(GAT)5 |
184 |
2 |
0.39 |
+ |
+ |
+ |
+ |
- |
+ |
- |
- |
+ |
- |
+ |
1 |
- |
EH791609.1 |
LM-74 |
CTTACCGTCCCCAGCCATAC |
(TCC)6 |
193 |
1 |
M |
+ |
+ |
- |
+ |
- |
+ |
+ |
+ |
+ |
+ |
+ |
1 |
- |
EH791602.1 |
LM-75 |
CCGCCGGAAAAAGAATTTAC |
(CAG)5 |
161 |
1 |
M |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
1 |
- |
EH791368.1 |
LM-76 |
GATATCGTCGACCCACGC |
(ATC)5 |
150 |
2 |
0.40 |
+ |
+ |
+ |
- |
+ |
+ |
+ |
- |
+ |
- |
+ |
2 |
- |
EH791352.1 |
LM-77 |
CTTTTGAAGCTATGGCGGAG |
(TTC)8 |
165 |
1 |
M |
+ |
- |
- |
- |
- |
- |
- |
- |
- |
- |
+ |
3 |
- |
EH791256.1 |
LM-79 |
GCAACAGCAGCAGTAAGCAG |
(CAT)5 |
173 |
2 |
0.18 |
+ |
+ |
+ |
+ |
+ |
+ |
- |
+ |
+ |
+ |
+ |
3 |
+ |
EH791220.1 |
LM-80 |
CCACAACCAAATGGGAAATC |
(ATC)6 |
156 |
2 |
0.42 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
3 |
+ |
EH791187.1 |
LM-81 |
TCAACACCTTCAACACCACC |
(CAG)5(GGA)5 |
154 |
1 |
M |
+ |
+ |
- |
+ |
- |
+ |
- |
- |
- |
- |
- |
1 |
- |
CA483361.1 |
LM-84 |
CTTCCCAAAGAAGACCCTCC |
(GAA)7 |
174 |
1 |
M |
- |
+ |
- |
+ |
- |
+ |
- |
- |
- |
- |
- |
2 |
- |
CA483453.1 |
LM-85 |
CACCCCTGCTGGAAACAG |
(ACA)5(ATA)6 |
185 |
2 |
0.15 |
+ |
+ |
+ |
+ |
- |
+ |
+ |
- |
+ |
- |
+ |
1 |
- |
CV478207.1 |
LM-86 |
ACCTTTCCCAACCATAACCC |
(ATA)6 |
185 |
2 |
0.08 |
+ |
+ |
+ |
+ |
+ |
+ |
- |
- |
+ |
- |
- |
5 |
+ |
CV478210.1 |
LM-87 |
GACTATGGCCTTTCAGCACC |
(CTT)10 |
169 |
2 |
0.22 |
+ |
+ |
+ |
+ |
- |
+ |
+ |
- |
+ |
- |
+ |
3 |
+ |
CV478252.1 |
LM-88 |
CCTGATCTCCATAACTTCCCC |
(CAA)6 |
151 |
1 |
M |
+ |
+ |
- |
+ |
- |
+ |
+ |
- |
- |
- |
+ |
3 |
+ |
CV478306.1 |
LM-89 |
GGGAGAGAAGGGATTGGAAG |
(TAG)6 |
156 |
1 |
M |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
4 |
+ |
Tranferability (%) | 92.8 |
92.8 |
53.7 |
78 |
41.5 |
75.6 |
58.5 |
39 |
78 |
34.1 |
70.7 |
L.b.: Linum bienne; L.p.: Linum pallescens; L.a.: Linum altaicum; L.g.: Linum grandiflorum; L.n.: Linum narbonense; L.l.: Linum lewissi: L.h.: Linum hirsutum; L.t.: Linum tenuifolium; L.s.: Linum strictum; L.ta.: Linum tauricum; L.f.: Linum flavum. M: Monomorphic markers in L. usitatissimum. n/e: not expected size allele in Linum species. * EST-SSR markers which amplify potential multi allele/loci in Linum species. Annealing temperature was set at 60°C. |
|