Fig. 1 Screening of colonies under white light and under fluorescence. Cells which are plated in semi-solid media and incubated to form discrete colonies are imaged under white light (Panel a and c) and fluorescence (Panel b and d), respectively. High- and low-producer clones are depicted by the red and yellow arrows, respectively. In monoclonal antibody producing colonies, precipitation is formed around the respective colonies due to the interaction between secreted humanized anti-C2 monoclonal antibodies from the clones and the capture antibody conjugated to FITC. Therefore, the greater the quantity of antibodies secreted, the higher the exterior fluorescent intensity displayed (Panel b and d). In fact, the precipitation around the high producer could be visible even under white light (Panel a).

Supported by UNESCO / MIRCEN network