|
| Table 1. Comparison of exterior fluorescence intensity (FU) and antibody productivity (µg/ml) of clones selected by Clone Pix FL system. In general, clones above 1000 FU secretes humanized anti-C2 antibody with at least five times superiority compared to that of clones below 1000 FU. However, no correlation was observed between exterior fluorescence intensity and antibody productivity for clones with more than 1000 FU. |
Clones |
Exterior |
Antibody |
|
<1000 |
|
Clone A1 |
273 |
0.6 |
Clone A2 |
508 |
0.8 |
Clone A3 |
573 |
0.6 |
Clone A4 |
870 |
0.4 |
Clone A5 |
937 |
0.6 |
|
1000-2000 |
|
Clone B1 |
1107 |
8.3 |
Clone B2 |
1511 |
6.0 |
Clone B3 |
1648 |
11.4 |
Clone B4 |
1749 |
11.0 |
Clone B5 |
1968 |
7.4 |
|
2000-3000 |
|
Clone C1 |
2173 |
11.2 |
Clone C2 |
2479 |
8.8 |
Clone C3 |
2786 |
11.0 |
Clone C4 |
2910 |
7.2 |
Clone C5 |
2935 |
5.0 |
|
>3000 |
|
Clone D1 |
3318 |
8.7 |
Clone D2 |
3545 |
1.4 |
Clone D3 |
3840 |
8.6 |
|
|
|