Seq.1 (sequencing primer SF: GATGCACCTGATGAAATGGG )
TTTTTAAATCCAATTCGGTTGAACGAGTTCTCAAAAATGAACAATAATTCTAGATTCCGAGATATCGGTAATGGGC
CCTAGAGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTA
ACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCT
CTACAAATGTGGTAAAATCGATAAGGATCCAGGTGGCACTTTTCGTGCGATCTGCATCTCAATTAGTCAGCAACCA
TAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACT
AATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTT
GGAGGCCTAGGCTTTTGCAAAAAGCTTGGCATTCCGGTACTGTTGGTAAAGCCACCATGGAAGACGCCAAAAACAT
AAAGAAAGGCCCGGCGCCATTCTATCCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGA
TACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGGACATCACTTACGCTGAGTACTTCG
AAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGA
AAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATT
TATAATGAACGTGAATTGCTCAACAGTATGGGCATTTCGCAGCCTACCGTGGTGTTCGTTTCCAAAAAGGGTTGCA
AAAAATTTTGAACGTGCAAAAAAGCTCCCAATCATCCAAAAAATTATTATCATGGATTCTAAAACGGATTACCAGG
GGATTCAGTCGATGTACACGTCGTCACATCTCATCTACCTTCCCGGTTTATGATACGATTTTGTGCAAGAGTCTTC
GATAGGACAAGACAATTGCACTGATCATGACCTCTCTGATCTACTGGACTGCTAGGTGCGACCTTGTCTCATAGAC
TGCCTGGCCGGTAGAAATTCTCGCC
Seq.2 (sequencing primer SR: AGGACAGGTG CCGGCAGCGC) CCTGGAAATAAGAGGTGAGGTGATCATCAGGTAATGGGGTACTGTCGACGATACCCAATGCTATCAGGAGGCACGT
TATTCAGCGATCTGTCTATTCGGTCATCATAGTTGCTGACTCCCGTCGGTAGATAACTACGATACGGAGGCTACAA
TCTGCCCAGTGCTGCATGATACGCGAGACCACGCTCACCGGCTCAGATTATCAGCATAACAGCAGCGAGGGCCGAG
CGCAGAGTGTCTGCACTTATCCGCTCCATCCAGTCTATAATGTGCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAA
TAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGC
TCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTC
CGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGT
CATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGA
CCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTG
GAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGC
ACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCA
AAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATC
AGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTC
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCG
CCGCAGCCGAACGACCGAGCGCAGCGAGTCAGAGCGAGTGGGG
Seq.3 pFila sequence
Base pair: 6486bp
HSV TK promoter 7-759
Chimeric intron 826-962
T7 RNA polymerase Promoter (-17 to +2) 1006-1024
T7 RNA polymerase transcription initiation site 1023
Rluc reporter gene 1034-1969
XbaI restriction site 1971-1976
ApaI restriction site 1995-2000
SV40 late polyadenylation signal(upstream) 2039-2240
SV40 promoter 2279-2481
Fluc reporter gene 2511-4163
SV40 late polyadenylation signal(downstream) 4195-4416
Beta-lactamase (AmpR) 4800-5660
pBR322 plasmid replication origin 5802-6445
1 AGATCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA
51 GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG
101 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC
151 CGCGATCCTC TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT
201 CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC GCGCGCCCCA
251 GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG
301 GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG
351 ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC
401 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC
451 AAACACCAGG ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA
501 GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC
551 CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT
601 CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA
651 ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG
701 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA
751 CCCGCTTAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG
801 CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC
851 AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC
901 TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT
951 TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA
1001 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGACTT CGAAAGTTTA
1051 TGATCCAGAA CAAAGGAAAC GGATGATAAC TGGTCCGCAG TGGTGGGCCA
1101 GATGTAAACA AATGAATGTT CTTGATTCAT TTATTAATTA TTATGATTCA
1151 GAAAAACATG CAGAAAATGC TGTTATTTTT TTACATGGTA ACGCGGCCTC
1201 TTCTTATTTA TGGCGACATG TTGTGCCACA TATTGAGCCA GTAGCGCGGT
1251 GTATTATACC AGACCTTATT GGTATGGGCA AATCAGGCAA ATCTGGTAAT
1301 GGTTCTTATA GGTTACTTGA TCATTACAAA TATCTTACTG CATGGTTTGA
1351 ACTTCTTAAT TTACCAAAGA AGATCATTTT TGTCGGCCAT GATTGGGGTG
1401 CTTGTTTGGC ATTTCATTAT AGCTATGAGC ATCAAGATAA GATCAAAGCA
1451 ATAGTTCACG CTGAAAGTGT AGTAGATGTG ATTGAATCAT GGGATGAATG
1501 GCCTGATATT GAAGAAGATA TTGCGTTGAT CAAATCTGAA GAAGGAGAAA
1551 AAATGGTTTT GGAGAATAAC TTCTTCGTGG AAACCATGTT GCCATCAAAA
1601 ATCATGAGAA AGTTAGAACC AGAAGAATTT GCAGCATATC TTGAACCATT
1651 CAAAGAGAAA GGTGAAGTTC GTCGTCCAAC ATTATCATGG CCTCGTGAAA
1701 TCCCGTTAGT AAAAGGTGGT AAACCTGACG TTGTACAAAT TGTTAGGAAT
1751 TATAATGCTT ATCTACGTGC AAGTGATGAT TTACCAAAAA TGTTTATTGA
1801 ATCGGACCCA GGATTCTTTT CCAATGCTAT TGTTGAAGGT GCCAAGAAGT
1851 TTCCTAATAC TGAATTTGTC AAAGTAAAAG GTCTTCATTT TTCGCAAGAA
1901 GATGCACCTG ATGAAATGGG AAAATATATC AAATCGTTCG TTGAGCGAGT
1951 TCTCAAAAAT GAACAATAAT TCTAGATTCC GAGATATCGG TAATGGGCCC
2001 TAGAGCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA
2051 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
2101 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
2151 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG
2201 GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA
2251 GGATCCAGGT GGCACTTTTC GTGCGATCTG CATCTCAATT AGTCAGCAAC
2301 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT
2351 CCGCCCATTC TCCGCCCCAT CGCTGACTAA TTTTTTTTAT TTATGCAGAG
2401 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT
2451 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TGGCATTCCG GTACTGTTGG
2501 TAAAGCCACC ATGGAAGACG CCAAAAACAT AAAGAAAGGC CCGGCGCCAT
2551 TCTATCCGCT GGAAGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG
2601 AAGAGATACG CCCTGGTTCC TGGAACAATT GCTTTTACAG ATGCACATAT
2651 CGAGGTGGAC ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG
2701 CAGAAGCTAT GAAACGATAT GGGCTGAATA CAAATCACAG AATCGTCGTA
2751 TGCAGTGAAA ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT
2801 TATCGGAGTT GCAGTTGCGC CCGCGAACGA CATTTATAAT GAACGTGAAT
2851 TGCTCAACAG TATGGGCATT TCGCAGCCTA CCGTGGTGTT CGTTTCCAAA
2901 AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAGCTCC CAATCATCCA
2951 AAAAATTATT ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA
3001 TGTACACGTT CGTCACATCT CATCTACCTC CCGGTTTTAA TGAATACGAT
3051 TTTGTGCCAG AGTCCTTCGA TAGGGACAAG ACAATTGCAC TGATCATGAA
3101 CTCCTCTGGA TCTACTGGTC TGCCTAAAGG TGTCGCTCTG CCTCATAGAA
3151 CTGCCTGCGT GAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA
3201 ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT
3251 TGGAATGTTT ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT
3301 TAATGTATAG ATTTGAAGAA GAGCTGTTTC TGAGGAGCCT TCAGGATTAC
3351 AAGATTCAAA GTGCGCTGCT GGTGCCAACC CTATTCTCCT TCTTCGCCAA
3401 AAGCACTCTG ATTGACAAAT ACGATTTATC TAATTTACAC GAAATTGCTT
3451 CTGGTGGCGC TCCCCTCTCT AAGGAAGTCG GGGAAGCGGT TGCCAAGAGG
3501 TTCCATCTGC CAGGTATCAG GCAAGGATAT GGGCTCACTG AGACTACATC
3551 AGCTATTCTG ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA
3601 AAGTTGTTCC ATTTTTTGAA GCGAAGGTTG TGGATCTGGA TACCGGGAAA
3651 ACGCTGGGCG TTAATCAAAG AGGCGAACTG TGTGTGAGAG GTCCTATGAT
3701 TATGTCCGGT TATGTAAACA ATCCGGAAGC GACCAACGCC TTGATTGACA
3751 AGGATGGATG GCTACATTCT GGAGACATAG CTTACTGGGA CGAAGACGAA
3801 CACTTCTTCA TCGTTGACCG CCTGAAGTCT CTGATTAAGT ACAAAGGCTA
3851 TCAGGTGGCT CCCGCTGAAT TGGAATCCAT CTTGCTCCAA CACCCCAACA
3901 TCTTCGACGC AGGTGTCGCA GGTCTTCCCG ACGATGACGC CGGTGAACTT
3951 CCCGCCGCCG TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA
4001 GATCGTGGAT TACGTCGCCA GTCAAGTAAC AACCGCGAAA AAGTTGCGCG
4051 GAGGAGTTGT GTTTGTGGAC GAAGTACCGA AAGGTCTTAC CGGAAAACTC
4101 GACGCAAGAA AAATCAGAGA GATCCTCATA AAGGCCAAGA AGGGCGGAAA
4151 GATCGCCGTG TAATTCTTGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA
4201 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
4251 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC
4301 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA
4351 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC
4401 CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG ATGCCCTTGA
4451 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC
4501 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT
4551 GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
4601 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
4651 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GGTGCGCGGA
4701 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
4751 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
4801 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
4851 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
4901 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA
4951 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG
5001 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
5051 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
5101 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
5151 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
5201 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC
5251 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG
5301 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
5351 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
5401 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
5451 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
5501 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG
5551 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT
5601 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
5651 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
5701 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
5751 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
5801 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
5851 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
5901 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
5951 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
6001 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
6051 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
6101 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
6151 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
6201 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
6251 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
6301 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
6351 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
6401 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
6451 TGGCCTTTTG CTGGCCTTTT GCTCACATGG CTCGAC |
