Figure 2. PCR amplifications of p27 gene fragments in two CTV isolates

Upper panel: 0.8% agarose gel electrophoresis of PCR products of p27 (a) gene fragment (459 bp) in 30 clones of T-300 CTV isolate (lanes 1 to 30). Lane 31: molecular weigth marker (pcDNAII/ DdeI/ XhoI: 1140, 758, 540, 409 and 166 bp, respectively). Lane 32: negative control PCR reaction. Lane 33: molecular weigth marker (pcDNAII/ HinfI/ HindIII: 1129, 517, 453, 396, 236, 120, 75, 65 and 22 bp, respectively).

Lower panel: 0.8% agarose gel electrophoresis of PCR products of p27 (b) gene fragment (281 bp) in 10 clones of T-312 CTV isolate (lanes 3, 5, 6, 8, 9, 10, 12, 14, 15, and 18). Lane 1: molecular weigth marker (pcDNAII/ DdeI: 1898, 540, 409, and 166 bp, respectively). Lane 21: molecular weigth marker (pcDNAII/ HaeIII: 657, 458, 434, 290, 272, 174, 142, 102, 80, 44, 40, 29 and 24, respectively). Lane 22: negative control PCR reaction.

Supported by UNESCO / MIRCEN network
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