Figure 1. Effects of jasmonic acid (JA) and AlCl₃ in different concentrations and for different exposure times on the accumulation in roots and release of scopolamine and hyoscyamine in hairy roots cultures of B. candida

Results are expressed as FW (g) and percentages of increase or decrease of scopolamine and hyoscyamine with respect to the mean of corresponding control. Each value represents the mean of three independent determinations. The calculated variation coefficients are between 1.17 and 3.12%.

(a) Accumulation of tropane alkaloids (time of exposure: 24 h). Control values: tropane alkaloids (in µmoles/gFW): scopolamine(0.04); hyoscyamine(1.18). FW (g): 0.30.

(b) Accumulation of tropane alkaloids (time of exposure: 48 h). Control values: tropane alkaloids (in µmoles/gFW): scopolamine (0.08); hyoscyamine(0.79). FW (g): 0.33.

(c) Release of tropane alkaloids (time of exposure: 24 h). Control values: tropane alkaloids (in µmoles/gFW): scopolamine (0.18); hyoscyamine(0.12). FW (g): 0.30.

(d) Release of tropane alkaloids (time of exposure: 48 h) Control values: tropane alkaloids (in µmoles/gFW): scopolamine (0.04); hyoscyamine(0.19). FW (g): 0.33.