Table 2. Main A. niger metabolic processes at 120 hours culture time. Reactions are expressed in carbon-mol basis.

R0

Glucose (out) è Glucose (c) + (1/6) H+ (c)

R1

Glucose (c) + (1/6) ATP (c) è 1 F6P (c)

R2

Glucose (c) + (1/6) ATP (c) + (1/6) H2 O è (5/6) Ru5P (c) + (1/3) NADPH2 (c) + (1/6) CO2

R3

Ru5P(c) + (1/10) NADH2 (c) + (1/10) H2O è (4/10) Erythritol (c) + (6/10) F6P (c) + (1/10) Pi (c )

R4

Ru5P (c) + (1/5) NADH2 (c) + (1/5) H2O è Arabitol (c) + (1/5) Pi (c)

R5

F6P (c) + (1/4) G3P (c) è (5/4) Ru5P(c)

R6

Glucose (c) + (1/6) ATP (c ) + (1/6) NADH2 (c ) + (1/6) H2O è Mannitol (c) + (1/6) Pi (c)

R7

F6P (c) + (1/6) ATP (c) è (1/2) G3P (c) + (1/2) DHAP (c)

R8

G3P (c) + (1/3) Pi (c) è Pyruvate (c) + (2/3) ATP (c) + (1/3) NADH2 (c) +(1/3) H2O

R9

DHAP(c) + (1/3) NADH2 (c) è Glycerol-P (c)

R10

Glycerol-P (c) è DAHP (c) + (1/3) FADH2 (m)

R11

Glycerol-P (c) + (1/3) H2O è Glycerol (c) + (1/3) Pi (c)

R12

Pyruvate(c) + (1/6) NADH2(c) + (1/6) ATP(c) +(1/6) H2O è Citrate(c) + (1/3) NADH2 (m) + (1/6) Pi (c)

R13

Pyruvate(c) + (1/3) Pi (m) è CO2 + (4/3) NADH2 (m) + (1/3)FADH2 (m) + (1/3)ATP(m) + (1/3) H+ (m)

R14

Pyruvate(c)+ (1/3) H2O è CO2 + (2/3) NADH2 (m) + (1/3) NADH2 (c) + (1/3) FADH2 (m) + (1/3) H+ (m)

R15

NADH2(m) + (1/2) O2 è H2O + 3 ATP (m)

R16

FADH2 (m) + (1/2) O2 è H2O + 2 ATP (m)

R17

NADH2 (m) + (1/2) O2 è H20

R18

Citrate (c) + (1/2a ) ATP (c) + (1/2) H+(c) è Citrate (out) + (1/2a ) Pi (c)

R19

ATP (c) + a H+ (c) + H2O è ADP ( c) + a H+ (out) + Pi (c)

R20

ATP (m) + b H+ (m) + H2O è ADP (m) + b H+ (c ) + Pi (m)

R21

ATP (m) + ADP (c) + H+ (m) è ATP (c) + ADP (m) + H+ (c )

R22

Pi (c) + H+ (c) è Pi (m) + 2 H+ (m)

R23

DHAP (c) è G3P (c)

R24

NADPH2 (c) + NAD+ (c) è NADP+ (c) + NADH2 (c)


R0: H+-symport Glucose carrier
R1:Hexokinase
R2: G-6P dehydrogenase + Gluconate-6P dehydrogenase
R3: Ru-5P isomerase + Erytrose reductase + Phosphatase
R4: Ru-5P isomerase + Reductase+ Phosphatase
R5: Transketolase + Transaldolase
R6: G-6P epimerase + Phosphatase
R7: Aldolase
R8:G-3P dehydrogenase + Enolase
R9: Glycerol-P dehydrogenase (c)
R10: Glycerol-P translocator + Glycerol-P dehydrogenase (m) + DAHP translocator
R11: Phosphatase
R12: H+-symport Pyruvate translocator [piruvate-1(c)/H+(c)] + Pyruvate descarboxilase(m) + Pyruvate kinase (c) + Malate dehydrogenase (c) + mitochondrial Citrate translocator [malate-2(c)/citrate-3(m), H+(m)] + Malate dehydrogenase(m) + Citrate synthase(m)
R13: H+-symport Pyruvate translocator [piruvate-1(c)/H+(c)] + Pyruvate decarboxilase(m) + Krebs cycle enzymes + mitochondrial transhydrogenase
R14: Pyruvate translocator [piruvate-1(c)/H+(c)] + Pyruvate decarboxilase (m) + Citrate synthase (m) + Aconitase (m) + IDH (NADP dependent) + KG dehydrogenase (m) + Glutamate dehydrogenase (m) + Translocator Dicarboxilic Acids (m)+ GABA cycle enzymes (c) + Succinate dehydrogenase (m) + Fumarase (m) + Malate dehydrogenase (m)
R15: Phosphorylation oxidative of NADH (m)
R16: Phosphorylation oxidative of FADH (m)
R17: Alternative respiration
R18: citrate translocator (c)
R19: cytoplasmatic H+-ATP-ase
R20: mitochondrial H+-ATPase
R21: ATP translocator [ATP-4(m), H+(m)/ADP-3(c)]
R22: H+-symport Phosphate carrier
R23:Triose-P isomerase
R24: cytoplasmatic transhydrogenase enzyme
(m): mitochondrial.
(c): cytoplasmatic
(out): extracellular.

 
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