Figure 2. HPLC chromatography. The immunoreactive molecules (fraction B) obtained from the Toyopearl column were loaded on C18 Alltech column. The line shows the concentration of acetonitrile in the eluting solvent. Optical density (¨) was measured at 226 nm and the CGRP like immunoreactivity (l) determined on an aliquot (0.4 ml) from each fraction.