Table 1 - Purification of a competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates

Table 1. Purification of CGRP like molecules obtained from sardine hydrolysates.

	Proteins, µg	CGRP-like immuno reactivity, pg	Specific activity^a, pg/µg protein	Purification factor^b	RRA ED_50, µg/tube^c
Crude extract	22,000	30	1.4 10^-4		311
Gel Exclusion	250	16	0.06	46	101
HPLC 1	54	23	0.42	304	7.3
HPLC 2	14	245	18	12500	0.9

^aThe specific activity represents the quantity of CGRP like molecules per µg of protein.

^b The purification factor is the ratio of the specific activity of the crude extract to that obtained at each purification step.

^cData were expressed as the quantity of proteins (µg) that induced a 50% inhibition of the initial CGRP binding to rat liver membranes.

Supported by UNESCO / MIRCEN network

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