Time series of neurite remodeling. Photomicrographs of a hippocampal pyramidal neuron expressing eGFP were obtained at 3 h intervals (top row: A, 0 h; B, 3 h; C, 6 h; D, 9 h). Image A was obtained 24 h post-inoculation with the viral vector. The digital images were inverted so that eGFP fluorescence is shown as black on a gray background. The bottom row shows subtraction images that reveal the degree of neurite remodeling. Images E-G are digitally subtracted images (E=B-A; F=C-A; G=D-A).

Objects that have shifted from their original position in A will appear black in these images, whereas the original position of objects in A will appear white. Note the degree of movement of the soma as well as the neurites.