Molecular Biotechnology and Genetics
 Marine Biotechnology
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.4 No. 2, Issue of August 15, 2001
© 2001 by Universidad Católica de Valparaíso -- Chile Received December 9, 2000 / Accepted July 10, 2001
RESEARCH ARTICLE

Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish (Danio rerio)

Reynold Morales
Mammalian Cell Genetics Division
Center for Genetic Engineering and Biotechnology
PO Box 6162, Havana 10600, Cuba
Tel: 537-216022
Fax: 537-331779
 E-mail: reynold.morales@cigb.edu.cu

María Teresa Herrera
Department of Animal and Human Biology
Faculty of Biology, University of Havana
25th street No. 455, Havana 10400, Cuba

Amílcar Arenal
Center for Genetic Engineering and Biotechnology
PO Box 387, Camagüey 1, Cuba
Tel: 537-216022
Fax: 537-331779
E-mail: amilcar.arenal@cigbcam.cigb.edu.cu

Asterio Cruz
Division of Quality Control and Assurance
Center for Genetic Engineering and Biotechnology
PO Box 6162, Havana, Cuba
Tel: 537-216022
Fax: 537-331779
E-mail: asterio.cruz@cigb.edu.cu

Oscar Hernández
Center for Genetic Engineering and Biotechnology
PO Box 387, Camagüey 1, Cuba

Rafael Pimentel
Center for Genetic Engineering and Biotechnology
PO Box 387, Camagüey 1, Cuba
Tel: 537-216022
Fax: 537-331779
E-mail: rafael.pimentel@cigbcam.cigb.edu.cu

Isabel Guillén
Mammalian Cell Genetics Division
Center for Genetic Engineering and Biotechnology
PO Box 6162, Havana, Cuba
Tel: 537-216022
Fax: 537-331779
E-mail: isabel.guillen@cigb.edu.cu

Rebeca Martínez
Mammalian Cell Genetics Division
Center for Genetic Engineering and Biotechnology
PO Box 6162, Havana, Cuba
Tel: 537-216022
Fax: 537-331779
E-mail: rebeca.martinez@cigb.edu.cu

Mario P Estrada*
Mammalian Cell Genetics Division
Center for Genetic Engineering and Biotechnology
PO Box 6162, Havana, Cuba
Tel: 537-218008 / 537-218466
Fax: 537-36008 / 537-218070
E-mail: mpablo@cigb.edu.cu

* Corresponding author

Keywords: carp, GFP, growth hormone, tilapia, transgenic, zebrafish.


Abstract Full Text

Gene transfer is economically important and model fish species has produced a great impact in modern biology and biotechnology. Transgenic zebrafish (Danio rerio) were generated through the co-injection of a GFP-expressing plasmid and an "all fish" transgene composed by the carp b-actin promoter and the chromosomal tilapia (Oreochromis hornorum) growth hormone gene. The GFP expression was a good indicator of stable transformation and allowed for high efficiency selection of transgenic fish. Transgenic F1 zebrafish grew 20% faster than full sibling non-transgenic controls.
Supported by UNESCO / MIRCEN network
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