Process Biotechnology
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.4 No. 2, Issue of August 15, 2001
© 2001 by Universidad Católica de Valparaíso -- Chile Received May 16, 2001 / Accepted July 17, 2001

Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize

Deise Maria Fontana Capalbo*
Embrapa Meio Ambiente
C.P. 69, CEP: 13820 000
Jaguariúna, São Paulo, Brasil
Tel: (19) 323333764
Fax: (19) 38678740

Fernando Hercule Valicente
Embrapa Milho e Sorgo
C.P. 151, CEP: 35701 970
Sete Lagoas, Minas Gerais, Brasil
Tel: (31) 7791000
Fax: (31)7791088

Iracema de Oliveira Moraes
Universidade Guarulhos
Praça Tereza Cristina, 01, CEP: 07023-070
Guarulhos, São Paulo, Brasil
Tel: (19) 32582001
Fax: (19) 32582001

Lúcia Helena Pelizer
Fundação de Amparo à Pesquisa do Estado de São Paulo Fellow
Tel: (16) 91211736
Fax: (16) 37227769

*Corresponding author

Financial Suport: FAPESP, CNPMA/BR and CNPMS/BR.

Bacillus thuringiensis, biological control, biopesticide, semi-solid fermentation, Spodoptera frugiperda.

Abstract Full Text

The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda (fall armyworm) in corn. More than 109 CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 106 spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days of spraying, and all larvae were found dead on leaves. During one maize crop cycle, two applications were made, and up until 70 days after emergence it was not necessary to apply any other insecticide for fall armyworm control.

  Supported by UNESCO / MIRCEN network
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