Environmental Biotechnology
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.4 No. 2, Issue of August 15, 2001
© 2001 by Universidad Católica de Valparaíso -- Chile Received May 11, 2001/ Accepted July 18, 2001
SHORT COMMUNICATION

Production of polyhidroxyalkanoates by Pseudomonas putida KT2442 harboring pSK2665 in wastewater from olive oil mills (alpechín)

Rafael Garcia Ribera*
Department of Microbiology, Faculty of Pharmacy
University of Granada
Campus Universitario de Cartuja, 18071
Granada, Spain
Tel: 34-958-249975
Fax: 34-958-246235
E-mail: rafaelgr@platon.ugr.es

Mercedes Monteoliva-Sanchez
Department of Microbiology, Faculty of Pharmacy
University of Granada
Campus Universitario de Cartuja, 18071
Granada, Spain
Tel: 34-958-243875
Fax: 34-958-246235
E-mail: mmonteol@platon.ugr.es

Alberto Ramos-Cormenzana
Department of Microbiology, Faculty of Pharmacy
University of Granada
Campus Universitario de Cartuja, 18071
Granada, Spain
Tel: 34-958-243877
Fax: 34-958-246235
E-mail: ramosca@platon.ugr.es

* Corresponding author

Financial support: Grants PB96-1403 and OLI-96-2189 from the Ministry of Education and Culture of Spain.

Keywords: biorremediation, olive oil mills wastewater, polyhidroxyalkanoates, Pseudomonas putida.

Abstract Full Text

The nutritionally versatile Pseudomonas putida has showed to grow in olive oil waste water called "alpechín", which is toxic for many other microorganisms. The transformation with the plasmid pSK2665, harboring Alcaligenes eutrophus genes needed for synthesis of poly (3-hidroxybutyric acid), allow Pseudomonas strain to grow in high concentration of the wastewaters "alpechín" accumulating biodegradable thermoplastic.
Supported by UNESCO / MIRCEN network
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