Process Biotechnology
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.4 No. 2, Issue of August 15, 2001
© 2001 by Universidad Católica de Valparaíso -- Chile Received April 24, 2001 / Accepted July 17, 2001

Production of natural and rare pentoses using microorganisms and their enzymes

Zakaria Ahmed
Food Science and Biochemistry Division
Faculty of Agriculture, Kagawa University
Kagawa 761-0795, Kagawa-Ken, Japan

Financial support: Ministry of Education, Science, Sports and Culture of Japan under scholarship program for foreign students.

Keywords: enzyme, microorganism, monosaccharides, pentose, rare sugar.

Present address: Scientific Officer, Microbiology and Biochemistry Division, Bangladesh Jute Research Institute, Shere-Bangla Nagar, Dhaka-1207, Bangladesh. Tel: 880-2-8124920.

Abstract Full Text

Biochemical methods, usually microbial or enzymatic, are suitable for the production of unnatural or rare monosaccharides. D-Arabitol was produced from D-glucose by fermentation with Candida famata R28. D-xylulose can also be produced from D-arabitol using Acetobacter aceti IFO 3281 and D-lyxose was produced enzymatically from D-xylulose using L-ribose isomerase (L-RI). Ribitol was oxidized to L-ribulose by microbial bioconversion with Acetobacter aceti IFO 3281; L-ribulose was epimerized to L-xylulose by the enzyme D-tagatose 3-epimerase and L-lyxose was produced by isomerization of L-RI. L-ribose and L-arabinose were prepared biochemically from ribitol by oxidation using Acetobacter aceti IFO 3281 and isomerization using L-RI and L-arabinose isomerase (L-AI), respectively. Other pentoses can be produced as well by cell or enzyme bioconversions.

Supported by UNESCO / MIRCEN network
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