Table
1. Selectable marker genes used in wheat transformation.
Gene |
Selective agent |
Mode of Action |
Reference |
pat, bar |
Phosphinothricin, bialaphos |
Inhibits glutamine synthase |
|
nptII |
Kanamycin, G418, paromomycin |
Binds 30S ribosomal subunit, inhibits translation |
|
hpt |
Hygromycin |
Binds 30S ribosomal subunit, inhibits translation |
|
EPSPS |
Glyphosate |
Inhibits aromatic acid biosynthesis (EPSPS) |
|
Glyphosate oxidoreductase (GOX) |
Glyphosate |
Degradation of glyphosate into aminomethyl phosphonic acid |
|
Mannose-6-phosphate |
Mannose |
Ability to grow on mannose as a sole carbon source |
|
Cyanamide hydratase (Cah) |
Cyanamide |
Ability to grow on cyanamide containing media, converts cyanamide into urea |
Abbreviations: |
|