Process Biotechnology
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.4 No. 3, Issue of December 15, 2001.
© 2001 by Universidad Católica de Valparaíso -- Chile Received April 9, 2001 / Accepted July 27, 2001
RESEARCH ARTICLE

Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier

Sarah Afaq
Research Scholar in Biochemistry
Department of Biochemistry, Faculty of Life Sciences
Aligarh Muslim University, Aligarh-202002 (U.P.), India
Tel: 091 571-700741

Jawaid Iqbal*
Senior Lecturer
Department of Biochemistry, Faculty of Life Sciences
Aligarh Muslim University, Aligarh-202002 (U.P.), India
Tel: 091 571-700741
E-mail: j.iqbal@angelfire.com

* Corresponding author

Financial support: Council of Scientific and Industrial Research (C.S.I.R.).

Keywords: papain, immobilized metal ion (IMI) carrier, immobilization, thermal stability, regeneration of matrix.


Abstract Full Text

A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity.
Supported by UNESCO / MIRCEN network
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