Figure 5. Proteases secreted by wt (lane 2) and mutant 7-121 of T. aureoviride (lane 3) and by isolate P6 of T. aureoviride (lane 4). Cultures were grown on chitin as carbon source. Native PAGE was carried out in 15%, polyacrylamide gels pH 8.8 in the presence of PMSF. Positive control is proteinase K (lane 1).