Molecular Biology and Genetics
EJB Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 5 No. 1, Issue of April 15, 2002
© 2002 by Universidad Católica de Valparaíso -- Chile Received October 8, 2001 / Accepted April 10, 2002
RESEARCH ARTICLE

Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene

Chuan-Bao Sun*#
 Laboratory of Genetic Engineering
Department of Bio-pharmaceutical
Shanghai Institute of Pharmaceutical Industry
Shanghai, 200040  P. R. China
E-mail: chuanbaosun@hotmail.com

Qiu-Lian Kong
 Department of Process and Store of Agro-products
Shanghai Academy of Agricultural Science
Shanghai, 201106  P. R. China
Tel: 0086 21 62208660 3077
E-mail: qiuliank@yahoo.com.cn

Wen-Si Xu
 Shanghai Institute of Pharmaceutical Industry
Shanghai, 200040  P. R. China
Tel: 0086 21 62472530

* Corresponding author

Financial support: Shangai Postdoctoral Financial Comitee.

Keywords: Agrobacterium tumefaciens,  Penicillium chrysogenum, Ti plasmid, transformation.

Present Address: # The Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Tel: 65 68 72 7491, Fax: 65 68 727007.

Abstract Full Text

The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts.

Supported by UNESCO / MIRCEN network
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