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Molecular Biology and
Genetics
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| EJB Electronic Journal of Biotechnology
ISSN: 0717-3458 |
Vol. 5 No. 1, Issue of April
15, 2002. |
| © 2002 by Universidad Católica de Valparaíso
-- Chile |
Received June 12, 2001 / Accepted April 10, 2002 |
Application of rice (Oryza
sativa L.) suspension culture in studying senescence in vitro
(II). Changes in DNA integrity
Ramin Hosseini *#
Plant
Research Group
YAPO School of Biological Sciences
University of Nottingham
University Park Nottingham NG7 2RD
United Kingdom
E-mail: Raminh_2001@Yahoo.com
Bernard J. Mulligan
§
Plant Research Group
School of Biological Sciences
University of Nottingham
University Park Nottingham NG7 2RD
United Kingdom
E-mail: Bernard.Mulligan@DG12.cec.be
* Corresponding author
Financial support:
Ministry of Science, Research and Technology of Iran.
Keywords:
Programmed Cell Death (PCD), rice, senescence.
Present address: #Research
Centre for Applied Sciences, Jahade Daneshgahi, Shahid Beheshti University,
Evin, Tehran, Iran. Tel: 0098-21-2402097 (ext. 213). Fax: 0098-21-9403884.
§European Commission, DG12, Office SDME 9/28, 200 Ruela Lai,
1049, Brussels, Belgium.
Changes in the DNA content
and organisation of senescing rice cell cultures (Taipei 309) were studied,
using PCR and Southern blot analyses. A mitochondrial gene (coxII),
a plastid gene (psaA) and a nuclear DNA maker (RG64) were
analysed. The amplification of mitochondrial (mt), plastid and nuclear
DNA produced the expected fragments, indicating that there were still
some intact organelles and nuclei in the senescing rice cells. However,
in plastid and nuclear DNA, changes in the number and size of the PCR
products were observed. Southern blot analysis revealed that mt and
nuclear DNA contents declined, but plastid DNA content remained relatively
the same throughout the senescence.
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