Abstract - Analysis of genetic diversity in the Oryza officinalis complex

Analysis of genetic diversity in the Oryza officinalis complex

María Teresa Federici*
Unidad de Biotecnologia
Instituto Nacional de Investigación Agropecuaria
Estación Experimental “Las Brujas”
Ruta 48, km 10, Rincón del Colorado
Canelones, Uruguay
Tel: 598 2 0327 7641
Fax: 598 2 0327 7609
E- mail: maritefe@inia.org.uy

Andrei B. Shcherban
Laboratory of Molecular Genetics of Cereals
Institute of Cytology and Genetics,
Lavrentieva Ave.10, 630090
Novosibirsk, Russia
E- mail: atos@bionet.nsc.ru

Fabián Capdevielle
Unidad de Biotecnologia
Instituto Nacional de Investigación Agropecuaria
Estación Experimental “Las Brujas”
Ruta 48, km 10, Rincón del Colorado
Canelones, Uruguay
Tel: 598 2 0327 7641
Fax: 598 2 0327 7609
E- mail: fabian@inia.org.uy

Marta Francis
Unidad de Biotecnologia
Instituto Nacional de Investigación Agropecuaria
Estación Experimental “Las Brujas”
Ruta 48, km 10, Rincón del Colorado
Canelones, Uruguay
Tel: 598 2 0327 7641
Fax: 598 2 0327 7609
E- mail: mfrancis@inia.org.uy

Duncan Vaughan
Crop Evolutionary Dynamics Laboratory
National Institute of Agrobiological Resources (NIAR)
Kannondai 2- 1- 2, Tsukuba
Ibaraki, 305, Japan
Tel: 81 298 38 7406
Fax: 81 298 389 7408
E- mail: duncan@abr.affrc.go.jp

* Corresponding author

Financial support: Japan Cooperation Agency – individual training of Group Course “Plant Genetic Resources”.

Keywords: dendrogram, genetic diversity, Oryza officinalis complex, RFLP´s.

The genetic relationships among 34 accessions of wild rice from Asia, Africa, America and Australia were analysed using RFLP technique. After southern blotting, DNA digestion pattern was hybridised with a highly repetitive DNA sequence of a retrotransposon from a gypsy family of mobile elements. A dendrogram was constructed from RFLP data in which the species clustered according to their genome designation (CC, BB, BBCC and CCDD genomes). Some species did not appear in the same group, for example, O. eichingeri from Africa and Sri Lanka clustered separately from each other. The same situation was observed for the accessions from China of O. officinalis, which cluster together showing a close relationship with O. rhizomatis, and O. eichingeri (both of CC genome). Also, the tetraploid BBCC from India of O. officinalis appears in the same cluster of O. eichingeri and O. punctata (both from Africa) suggesting close phylogenetic relationship with the African genomes BB, CC and BBCC.