Table 1. Strains and plasmids
used in this work.
Strain/plasmid |
Relevant phenotype/genotype |
Source |
R.eutropha JMP134 (pJP4) |
2,4-D, 3-CB |
DSMZ |
R. eutropha JMP222 |
Smr |
H. Knackmuss |
P. putida KT2442 |
Rfr |
|
E. coli CC118lpir |
Rfr |
|
E. coli HB101 (pRK600) |
Cmr, tra+ |
|
A. lwoffii RB2 |
Gu |
this work |
pUT |
Kmr, Apr |
|
pCNB5 |
Kmr, Apr, lacIq/Ptrc |
|
pITFD, pCNB5 derivative |
Kmr, Apr, lacIq /Ptrc tfdICDEF |
this work |
pDTFD, pUT derivative |
Kmr, Apr, tfdR/Ptfd-I tfdICDEF |
this work |
pTFD, pUT derivative |
Kmr, Apr, Ptfd-I tfdICDEF |
this work |
2,4-D,
3-CB, Gu: able to grow on 2,4-dichlorophenoxyacetate, 3-chlorobenzoate,
or guaiacol; tra+: IncP transference functions; tfd:
catabolic genes from pJP4; lacIq: lac repressor
mutant; Ptrc: lac/trp hybrid promoter; tfdR:
regulatory gene of pJP4; Ptfd-I: promoter region for tfdICDEF
cluster; Ap: ampicillin; Km: kanamycin; Rf: rifampycin; Cm: cloramphenicol;
Sm: streptomycin. DSMZ: Deutsche Sammlung von Mikroorganismen und
Zellkulturen. |
|