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Table 2. Catabolic characterization
of A. lwoffii RB2.
Substrate |
Growth |
% Dechlorination* |
| Phenol |
+ |
n.a.** |
| 3-chlorophenol |
- |
0 |
| 4-chlorophenol |
- |
6 |
| 2,4-dichlorophenol |
- |
0 |
| Benzoate |
+ |
n.a. |
| 2-chlorobenzoate |
- |
0 |
| 3-chlorobenzoate |
- |
20 |
| 4-chlorobenzoate |
- |
0 |
| 2,4-dichlorobenzoate |
- |
0 |
| 3,5-dichlorobenzoate |
- |
0 |
| Guaiacol |
+ |
n.a. |
| 4-chloroguaiacol |
- |
28 |
| 5-chloroguaiacol |
- |
95 |
| 6-chloroguaiacol |
- |
0 |
| 3,5-dichloroguaiacol |
- |
0 |
| 4,5-dichloroguaiacol |
- |
0 |
| 4,6-dichloroguaiacol |
- |
0 |
| Catechol |
- |
n.a. |
| 3-chlorocatechol |
- |
0 |
| 4-chlorocatechol |
- |
10 |
| 3,5-dichlorocatechol |
- |
11 |
| *% Dechlorination: calculated from the stoichiometric theoretical value. Growth tests were carried out in minimal medium liquid cultures with 0.2-2 mM carbon source and incubated for up to 10 days. For dechlorination assays cells were pre-grown on the corresponding non-chlorinated substrate. In the case of chlorocatechols cells were pre-grown on 2 mM benzoate. Dechlorination was determined after 1 day with 1 mM substrate. **n.a.: non applicable. |
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