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| Table 3. | Tfd enzyme activities and rates of 3-CB dechlorination in R. eutropha, P. putida and A. lwoffii strains. |
| Strain/clone |
Inducer |
CC-1,2- DO (U/mg) |
CMCI |
3-CB dechlorination rate (nmols/min/mg) |
| R.eutropha JMP134 |
3-CB |
0.51 |
0.08 |
37 |
| R.eutropha JMP134 |
- |
0.04 |
0.00 |
19 |
| R.eutropha JMP222 |
3-CB |
0.00 |
0.00 |
0 |
| R. e. JMP222::ITFD5 |
- |
0.03 |
0.00 |
2 |
| R. e. JMP222::ITFD5 |
IPTG |
0.40 |
0.03 |
31 |
| R. e. JMP222::TFD5 |
- |
0.03 |
0.00 |
1 |
| R. e. JMP222::TFD5 |
3-CB |
0.02 |
0.00 |
1 |
| R. e. JMP222::DTFD4 |
- |
0.03 |
ND |
1 |
| R. e. JMP222::DTFD4 |
3-CB |
0.32 |
ND |
18 |
| P. putida KT2442 |
3-CB |
0.00 |
0.00 |
0 |
| P. p. KT2442::ITFD1 |
- |
0.02 |
0.00 |
0 |
| P. p. KT2442::ITFD1 |
IPTG |
0.22 |
0.02 |
23 |
| P. p. KT2442::TFD7 |
- |
0.13 |
0.01 |
20 |
| P. p. KT2442::TFD7 |
3-CB |
0.08 |
0.01 |
12 |
| A. lwoffii RB2 |
- |
0.00 |
0.00 |
0.4 |
| A. l. RB2::ITFD3 |
- |
0.00 |
0.00 |
1 |
| A. l. RB2::ITFD3 |
IPTG |
0.01 |
0.00 |
2 |
Values are averages from two independent
measurements. Deviations were less than 10%, and 15%, for determinations
of enzyme activities, and dechlorination rates, respectively. Growth
substrate concentration was always 3 mM benzoate. CC-1,2-DO: Chlorocatechol-1,2-dioxygenase.
CMCI: Chloromuconate cycloisomerase. 3-CB: 3-chlorobenzoate. IPTG:
Isopropyl-beta-D-thiogalactopyranoside. |
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