Spirulina platensis growth estimation by pH determination at different cultivations conditions Departamento Tecnología Bioquímico-Farmaceutica Universidade de Franca Av. Dr. Armando Salles Oliveira 201 Parque Universitario, CEP 14404-600 Franca/ Sao Paulo, Brasil Tel: 55 16 37118888 Fax: 55 16 37118889 E-mail: lucia.pelizer@uol.com.br J.C.M.
Carvalho Sunao
Sato Laboratório de Bioprocessos Universidade Guarulhos Praça Thereza Cristina 01, Centro Guarulhos CEP 07023070 Sao Paulo, Brasil Tel: 55 19 32582001 Fax: 55 19 32582001 E-mail: iomoraes@hotmail.com * Corresponding author Financial support: FAPESP. Keywords: biomass estimation, methodology, pH, protein content, Spirulina platensis.
Spirulina platensis is a cyanobacterium that has a high protein content and therefore, a high nutritional value. It can be cultivated either in a liquid or in a solid culture. When cultivated in aqueous culture the cell growth can be determined by following the optical density. On the other hand, when produced by solid cultivation the growth can be determined only indirectly, such as, through determination of the protein content of the fermenting solids. In this work the possibility of estimating cell growth by pH determination was verified. From the results it was concluded that pH and protein production (solid or surface culture) or cell content (liquid culture) correlate well, therefore pH determination seems to be a good method to determine cell growth. |
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