Table 2. Screening of molasses media obtained from different Pakistani Sugar Mills by Aspergillus niger GCBT7. All the fermentations were carried out at 30ºC following growth on 150 g/l initial sugar concentration. The initial pH of the molasses medium was kept constant at 6.0 throughout the fermentation period of 6-days.

Sugar Mills

Citric acid anhydrous (g/l)

% Yield*

Mycelial morphology

Madina Sugar Mills

57.60

61.54

Small round pellets

Pattoki Sugar Mills

46.15

54.87

Fine pellets

Kamalia Sugar Mills

85.56

91.90

Intermediate pellets

Premier Sugar Mills

77.65

88.32

Mixed pellets

Rahwali Sugar Mills

34.68

43.70

Gummy mass

Chunian Sugar Mills

62.55

75.24

Mixed broken mycelia

* based on the sugar consumed.


Supported by UNESCO / MIRCEN network