Table 1.
Influence of sucrose and nitrogen (as (NH4)2SO4) concentration on the growth and plumbagin production of Plumbago rosea roots culture.

 Experimental conditions

Biomass/litre
(g fresh weight  ±  SEM)

Plumbagin content
(% DW  ± SEM)

Sucrose concentration (g/l)

10.0

4.92 ± 0.5673

0.023 ± 0.0017

20.0

5.809 ± 0.5673

0.020 ± 0.0044

30.0 *

7.662 ± 0.5673

0.016 ± 0.0030

40.0

7.605 ± 0.5673

0.015 ± 0.0023

(NH4)2SO4 concentration (mM)

0.000

6.369 ± 0.5673

0.017 ± 0.0017

0.507

6.011 ± 0.8610

0.015 ± 0.0012

1.015*

6.001 ± 0.5243

0.016 ± 0.0030

2.030

6.567 ± 0.9557

0.020 ± 0.0015

*concentration used in basic B5 medium

Supported by UNESCO / MIRCEN network