Figure 1 - Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems

ELISAs for rhGM-CSF. Dilution curves in assay solution (PBS‑5% V/V FCS) for the samples of the rhGM‑CSF prepared in different media: □ PBS‑5% V/V FCS; ● cell culture medium - 0.1% V/V FCS; ▼ cell culture medium - 5% V/V FCS; Δ conditioned cell culture medium; ◊ LB medium; * conditioned LB medium.

	a) Sandwich ELISA with two MAbs; b) Sandwich ELISA with monoclonal and polyclonal antibodies; c) Competitive ELISA.
Figure 1.	ELISAs for rhGM-CSF. Dilution curves in assay solution (PBS‑5% V/V FCS) for the samples of the rhGM‑CSF prepared in different media: □ PBS‑5% V/V FCS; ● cell culture medium - 0.1% V/V FCS; ▼ cell culture medium - 5% V/V FCS; Δ conditioned cell culture medium; ◊ LB medium; * conditioned LB medium.