Figure 2.

a-b: Effect of voltage in the electroporation of coffee tissues without enzymatic treatment.

c-d: Effect of voltage in the electroporation of coffee tissues with 24 hours of enzymatic treatment.

e-f: Effect of temperature incubation with DNA, previously to the electroporation, without enzymatic treatment.

g-h: Effect of temperature incubation with DNA, previously to the electroporation, with 24 h enzymatic treatment.

c = calli; L = leaf; Et = torpedo shape somatic embryos; Eg = Globular shape somatic embryos.

375 V/cm
625 V/cm
875 V/cm
Temperature 0ºC
room
37ºC


Supported by UNESCO / MIRCEN network