Table
1. Regenerative response of electroporated torpedo somatic embryos.
|
Media conditions for regeneration |
Time of enzymatic treatment (hour) |
Voltage (V/cm) |
Dark brown % |
Explants with white calli % |
Explants with formation of secondary somatic embryos % |
Solid |
1 |
375 |
100.0 |
30.8 |
0 |
Solid |
1 |
625 |
100.0 |
14.8 |
0 |
Solid |
1 |
875 |
100.0 |
0 |
0 |
Solid |
24 |
375 |
100.0 |
7.5 |
0 |
Solid |
without |
375 |
100.0 |
27.0 |
0 |
Liquid |
1 |
375 |
77.3 |
0 |
43.2 |
Liquid |
1 |
625 |
80.0 |
0 |
16.0 |
Liquid |
1 |
875 |
100.0 |
0 |
0 |
Liquid |
24 |
375 |
71.4 |
0 |
0 |
Liquid |
without |
375 |
100.0 |
0 |
30.8 |
|