Table 1. Regenerative response of electroporated torpedo somatic embryos.

Media conditions for regeneration

Time of enzymatic treatment (hour)

Voltage (V/cm)

Dark brown %

Explants with white calli %

Explants with formation of secondary somatic embryos %

Solid

1

375

100.0

30.8

0

Solid

1

625

100.0

14.8

0

Solid

1

875

100.0

0

0

Solid

24

375

100.0

7.5

0

Solid

without

375

100.0

27.0

0

Liquid

1

375

77.3

0

43.2

Liquid

1

625

80.0

0

16.0

Liquid

1

875

100.0

0

0

Liquid

24

375

71.4

0

0

Liquid

without

375

100.0

0

30.8

 


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