Electronic Journal of Biotechnology ISSN: 0717-3458
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Vol. 6 No. 2, Issue of August 15, 2003 |
© 2003 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received December 4, 2002 / Accepted April 24, 2003 |
Immobilized lysozyme protein on fibrous medium: Preliminary results
for microfiltration applications
Odilio B. G. Assis*
Embrapa
Agricultural Instrumentation Center
Caixa Postal 741, 13560-970
São Carlos, SP., Brazil
Tel: 55 16 2742477
Fax: 55 16 2725958
E-mail: odilio@cnpdia.embrapa.br
Luis C. Claro
PPG
Interunidades - Universidade de São Paulo USP
Caixa Postal
369, 13560-970
São Carlos,
SP., Brazil
Tel: 55
16 2742477
Fax: 55
16 2725958
E-mail:
lclaro@cnpdia.embrapa.br
*
Corresponding author
Financial support:
Embrapa, FAPESP and CNPq.
Keywords: enzyme
immobilization, packed bed column, surface analysis.
The
protein lysozyme was deposited onto a permeable support comprising
chemically functionalized glass fiber. The main objective of this
study was to set a stable organic net with no effect on the medium
bed permeability and a preliminary test the activity of this enzyme
under immobilized conditions. The film formation is followed by atomic
force microscopy (AFM) surface imaging. The effect on the bacteria
Escherichia coli was tested using a simple microfiltration
column. The filtration results pointed around 75% removal of bacteria
in the effluent when compared to the influent concentration. The removal
mechanism is assumed as being essentially due biointeraction. The
surface polarity characteristics of the formed film were also considered
as playing an important role, suggesting an electrostatic interaction
mechanism in the microorganism removal.
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