| Process
Biotechnology |
Biotechnology
Industry |
| Electronic Journal of Biotechnology
ISSN: 0717-3458 |
Vol. 6 No. 2, Issue of August 15, 2003 |
| © 2003 by Pontificia Universidad Católica
de Valparaíso
-- Chile |
Received January
8, 2003 / Accepted July 18, 2003 |
Optimization of b-xylosidase recovery by reversed
micelles using response surface methodology
Francislene
Andreia Hasmann*#
Instituto de Pesquisas
Tecnológicas
Butantan, University
of São Paulo
P.O. Box 66083
São
Paulo, Brazil
Tel: 55 11 30913862
Fax: 55 11 30913862
E-mail: fhasmann@uol.com.br
Daniela Vieira Cortez
Departamento de Biotecnología
Faculdade
de Engenharia Química
de Lorena
P.O. Box 116
Lorena,
São Paulo, Brazil
Tel: 55 12 5533165
Fax: 55 12 5533165
E-mail: dvcortez1@hotmail.com
Adalberto Pessoa Júnior
Departamento
de Tecnología Bioquímico-Farmacêutica
University
of São Paulo
P.O. Box 66083
São
Paulo, Brazil
Tel: 55 11 30913862
Fax: 55 11 30913862
E-mail: pessoajr@usp.br
Inês Conceição Roberto
Departamento
de Biotecnología
Faculdade de Engenharia
Química
de Lorena
P.O. Box 116
Lorena,
São Paulo, Brazil
Telephone: 55 12 5533165
Fax: 55 12 5533165
E-mail: ines@debiq.faenquil.br
*Corresponding Author
Financial support: FAPESP, CNPq and CAPES, Brazil
financially supported this work.
Keywords: b-xylosidase, experimental design, liquid-liquid extraction,
optimization, reversed micelles.
Present address: #Department of Biotechnology,
FAENQUIL, P.O. Box 116, São Paulo, Brazil, CEP 12600-000; Brazil;
Telephone: 55 12 5533165; Fax: 55 12 5533165. E-mail: francislene@debiq.faenquil.br
Abbreviations:
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ANOVA: analysis of variance;
CTAB: Cetyl Trimethyl Ammonium Bromide;
Da: Dalton;
pI: isoelectric point;
Rm: micellar radius;
SDS: sodiumdodecyl sulfate.
Wo: water in oil;
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b-Xylosidase
recovery by reversed micelles using CTAB cationic surfactant
was performed under different experimental conditions. A 22-full
orthogonal design with center points was employed to optimize
the recovery of this enzyme and to evaluate the influence of
the factors CTAB (A) and butanol concentration (B) on the enzyme
extraction. A mathematical model to represent the enzyme
recovery as a function of A and B was proposed.
According to the model, the CTAB and butanol concentrations
necessary to attain the maximum b-xylosidase recovery
(43%) were 0.26 M and 29.4%, respectively. The results showed
that the recovery value predicted by the model was similar to
that obtained experimentally (44.3%).
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