Table 1 - Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts

Table 1. Copies of enzyme gene transcripts detected from cold-exposed and control plants from wheat line 443 using quantitative real-time PCR.

Proportion of template RNA^a from plant treatment		Copies of transcript^b from gene encoding^c
Control	Cold-exposed	MnSOD	PLD
1	0	358447	79541
0.67	0.33	649536	131572
0.50	0.50	793987	144918
0.33	0.67	986075	160598
0.20	0.80	1103244	184888
0	1	1263288	216787
Mixed plant tissue		994767	183935
LSD^d		105480	23888

^aTotal RNA amount = 125ng.
^bMeans of three replications.
^cMnSOD = Manganese superoxide dismutase, PLD = phospholipase D.
^dLeast significant difference at P=0.05