Figure 1. Substrate specificity of N-glycosylated LsdA. TLC of products of levansucrase reaction on 100 mM sucrose (S), melezitose (Mel) or raffinose (R) in buffer NaAc 100 mM, pH 5.5, incubated at 30ºC for 10 hours in the absence A) or presence B) of 5 IU of enzyme. The enzymes studied were: LsdA expressed by P. pastoris (Pp), natural host Gluconacetobacter diazotrophicus (Gd) or recombinant E. coli strain (Ec). Other symbols represent: (K) kestose, (L) levan and (M) sugars marker.