Figure 3. Influence of temperature and substrate concentration on the transfructosylation reaction of glyco-LsdA. Reactions were conducted in buffer NaAc 100 mM, pH 5.5 with 5 IU of LsdA expressed by P. pastoris (Pp), natural host G. diazotrophicus (Gd) or recombinant E. coli strain (Ec) for 10 h at 42ºC and sucrose concentrations of 146, 584 and 1460 mM A) or temperatures of 60, 28 or 42ºC and 1460 mM of sucrose B). Sugars were separated by TLC. Symbols represent: (F) fructose, (S) sucrose, (K) kestose, (L) levan and (M) sugars marker.