Figure 4. Initial rate kinetics of the hydrolysis reaction catalysed by LsdA. Hydrolysis reaction was carried out in a sucrose concentration range of 1 to 50 mM at 60ºC for 2 minutes in the presence of 29.2 nM of LsdA expressed by P. pastoris (▼), natural host G. diazotrophicus (O) or recombinant E. coli strain (□), respectively. Fructose and glucose were measured as described in methods. The initial rates were determined on the basis of five independent measures and gathered data were statistically analyzed by non-linear regression using the Origin^TM Version 5 (Microcal^TM) computer software.