Figure 5. Effect of NaCl concentration on the transfructosylation reaction of LsdA. Sucrose at concentrations of 100 mM A) and 1500 mM B) was incubated for 10 hours in buffer NaAc 100 mM, pH 5.5, in the presence of 10 IU of LsdA expressed by P. pastoris (Pp), natural host G. diazotrophicus (Gd) or recombinant E. coli strain (Ec), at the indicated NaCl concentration and temperature. Product profiles were analyzed by TLC. Symbols represent: (F) fructose, (S) sucrose, (K) kestose, (L) levan and (M) sugars marker.