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Plant regeneration of Anthurium andreanum cv Rubrun Teresa
E. Vargas Alexander
Mejías Maira
Oropeza* Eva
de García *Corresponding author Financial support: This work was supported by the Council for Scientific and Humanistic Development (CDCH), Central University of Venezuela grant PI 0333-4393-2002 to Maira Oropeza. Keywords: Callus, micropropagation, organogenesis. Abbreviations:
BA: N6-benzyladenine;
To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 mM BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 mM BA and 0.05 mM NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 mM BA and 2.7 mM NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80% of plant acclimatization was obtained. |
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