Figure 3. Proteolytic degradation of MB-1TrpHis and mutants by E. coli proteases. At variance with results in Figure 2, proteolysis was performed after separating MB-1TrpHis mutants from its fusion partner MBP. Symbols: MB-1TrpHis; E44V45; E44M45. The average standard deviation for undegraded protein measurements is 5%. |
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