Figure 3. Estimation of molecular weights of GLOD subunits by SDS-PAGE in 3 different polyacrylamide separating gel concentrations, i.e. 10%, 12% and 14% with 4% stacking gel. The molecular weight markers used were: A, lysozyme (14,400); B, b-lactoglobulin (18,400); C, restriction endonuclease Bsp981 (25,000); D, lactate dehydrogenase (35,000); E, ovalbumin (45,000); F, bovine serum albumin (66,200)and G,b-galactosidase (116,000).