Figure 4. Isoelectric point determination of GLOD by isoelectric focusing. The standard pI markers were: A, amyloglucosidase (3.5); B, soybean trypsin inhibitor (4.55); C, b-lactoglobulin A (5.20); D, bovine carbonic anhydrase B (5.85); E, human carbonic anhydrase B (6.55); F, myoglobin-acidic band (6.85); G, myoglobin-basic band (7.35); H, lentil lectin-acidic band (8.15); I, lentil lectin-middle band (8.45); J, lentil lectin-base band (8.65); K, trypsinogen (9.30).