Figure 5. Effect of pH on GLOD activity (a) and stability (b).

(a) GLOD activity was assayed in various buffers with different pH values.

(b) (a) The enzyme was assayed in various buffers at different pH values by the method as described in the text.

(b) The enzyme was incubated in various buffers at the pH values indicated.

After incubation at 37ºC for 30 min, the remaining activities were determined by the method described in the text. The buffers (0.1 M) used were: acetate buffer (pH 3.5-6.0) (l), potassium phosphate buffer (pH 6.0-7.5) (u), Tris-HCl buffer (pH 7.5-9.0) (p), and glycine-NaOH buffer (pH 9.0-10.0) (n).