Purification step

Total protein (mg)

Total activity (U)

Specific activity
(U mg^-1)

Yield
(%)

Purification
(fold)

Culture extract

Ammonium sulphate

SP-SepharoseFF

Q -SepharoseFF

Gel filtration

1,203

376.6

10.7

0.72

0.2

185.25

151.03

70.23

33.43

30.85

0.15

0.4

6.56

46.48

152.36

100

81.53

37.91

18.05

16.65

1.0

2.6

42.7

302

990