Table 2. Substrate specificity of GLOD. The enzyme activity was measured as described in text. All values represent the percentage activity compared with the value obtained from L-glutamate. UD = undetectable.

Substrate (10 mM)

Relative activity (%)

L-glutamate

100

D-glutamate

0.79

L-glutamine

UD

L-aspartate

0.53

L-glycine

UD

L-alanine

UD

L-arginine

UD

L-cysteine

UD

L-histidine

UD

trans-4-hydroxy-L-proline

UD

L-lysine hydrochloride

UD

L-proline

UD

L-serine

UD

L-threonine

UD

L-valine

UD

L-isoleucine

UD

L-methionine

UD

L-phenylalanine

UD

L-leucine

UD

L-tryptophan

UD

L-tyrosine (1 mM)

UD

 

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