Table 2. Potential substrate consumption and FPase production parameters following growth of C. flavigena on a-cellulose-, CMC- or kallar grass-Dubos salts culture medium (pH 7.3-7.4) in shake-flask experiments conducted at 30ºC. The organism was grown on carbon sources (containing 1% carbohydrates in basal Dubos medium. Kallar grass (Leptochloa fusca) is a salt tolerant grass grown on saline lands, (Rajoka and Malik, 1997). The values in columns have been calculated from means of three replicates; the standard deviations were 2.5-3% of these values and have not been presented. The values of CBH were not significantly different from those for FPase and have been omitted.

Parameters

Following growth on 1% carbohydrates in

 

a-cellulose

CMC

kallar grass

YX/S  (g cells/g)

0.550

0.450

0.540

QS  (g S utized/I/h)

0.145

0.135

0.121

QX  (g cells/I/h)

0.240

0.200

0.234

QRS  (mg/I/h)

14.000

34.567

20.25

QP  (IU/I/h)

35.000

22.00

32.25

YP/S  (IU/g substrate consumed)

133.200

64.500

125.25

YP/X  (IU/g cells)

244.18

144.400

235.23

qP  (IU/g cells/h)

36.6

17.7

32.8

 

Supported by UNESCO / MIRCEN network