Table 5. Thermodynamic parameters estimated by Arrhenius approach for batch formation and deactivation of CBH by cultures of Cellulomonas flavigena. Thermodynamic parameters were determined using following equation: Ln (qp /T) = Ln(kB/h)+ ∆S*/R-∆H*/R.1/T ; where qp, T, kB , h, ∆S*, ∆H* and R are specific productivity, absolute temperature, Boltzmann constant, Planck's constant, entropy of activation, enthalpy of activation and gas constant respectively. The values of kB , h, and R are 1.38x10-23 J.K-1, 6.63 x 10-34 J.s and 8.314 J/.K-1.mol-1 respectively. ∆H* was calculated as slope and Ln(kB/h)+ ∆S*/R as intercept on Y-axis using Figure 2b. |
Enzyme formation |
Thermal inactivation |
|
Activation
enthalpy |
86.9 ± 3b |
92.6 ± 8a |
Activation
entropy |
90.5 ± 5a |
(-) 498.5 ± 25b |
|