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Table 2. Transient expression of GUS activity in immature embryos and plants derived from bombarded immature embryos. (a). Influence of macrocarrier gap on transient transformation frequency on bombarded immature embryos. Data collated from 9 replicates made of 50-70 embryos per bombardment for each macrocarrier gap evaluated. Transient transformation frequency was scored based on immature embryos showing gus expression 3 days after bombardment. |
|
Macrocarrier gap (mm) |
% transient transformation |
Kmr plants |
% Kmr plants with gus activity |
| 6 |
56.43 ±30.67 |
20.78 ±17.89 |
4.03 ±3.82 |
| 11 |
67.55 ±17.84 |
32.89 ±13.90 |
44.92 ±29.69 |
| 16 |
65.51 ±15.63 |
51.67 ±31.87 |
22.79 ±13.47 |
(b). Influence of helium pressure on transient transformation frequency on bombarded immature embryos. Data collated from 9 replicates made of 50-70 embryos per bombardment for each helium pressure evaluated. Transient transformation frequency was scored based on immature embryos showing gus expression 3 days after bombardment. |
|
Helium pressure (psi) |
% transient transformation |
Kmr plants |
% Kmr plants with gus activity |
| 900 |
67.36 ± 17.41 |
44.89 ± 25.38 |
31.31 ± 22.42 |
| 1100 |
59.32 ± 24.28 |
16.83 ± 13.76 |
14.66 ± 30.90 |
| 1300 |
59.32 ± 27.80 |
31.67 ± 23.04 |
23.09 ± 27.58 |
(c). Influence of embryo size on transient transformation frequency on bombarded immature embryos. Data collated from 9 replicates made of 50-70 embryos per bombardment for each embryo size evaluated. Transient transformation frequency was scored based on immature embryos showing gus expression 3 days after bombardment. |
|
Embryo size (mm) |
% transient transformation |
Kmr plants |
% Kmr plants with gus activity |
| < 3 |
68.98 ± 26.61 |
22.33 ± 15.80 |
14.70 ± 18.27 |
| 3-6 |
49.67 ± 26.57 |
22.50 ± 14.43 |
39.96 ± 38.96 |
| > 6 |
67.67 ± 10.13 |
43.11 ± 26.71 |
20.96 ± 19.15 |
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