Table 2. Single-cell sampling.

Methods

Advantages

Disadvantages

Flow-cytometric cell sorting

Large quantity of cells; rapid separation

Limit in some cells such as neuron without microenvironment data

Laser-assisted mechanical microdissection

Very little contamination with microenvironment data

Laborious

Laser-catapult microdissection

Very little contamination with microenvironment data

Special slides

Laser-capture microdissection

Very little contamination with microenvironment data

Theoretical damage to the target cell


Supported by UNESCO / MIRCEN network