Table 2. Single-cell sampling. |
Methods |
Advantages |
Disadvantages |
Flow-cytometric cell sorting |
Large quantity of cells; rapid separation |
Limit in some cells such as neuron without microenvironment data |
Laser-assisted mechanical microdissection |
Very little contamination with microenvironment data |
Laborious |
Laser-catapult microdissection |
Very little contamination with microenvironment data |
Special slides |
Laser-capture microdissection |
Very little contamination with microenvironment data |
Theoretical damage to the target cell |
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