|
|
Table 3. Functional genomics analysis for single cell or small cell number. |
Methods |
Minimum Cell |
Throughput |
Sequencing |
Application |
Disadvantage |
rtPCR base |
|||||
Multiplex rtPCR |
single cell |
poor |
N/A |
specific gene |
one cell <24 genes |
In situ rtPCR |
single cell |
poor |
N/A |
specific gene |
one cell <5 genes |
Gel base |
|
|
|
|
|
DD |
single cells |
medium |
low |
Parallel gene expression |
false positive |
Colony base |
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Subtractive cloning |
100-104 cells |
medium |
low |
Parallel gene expression |
not typical parallel |
Hybridization base |
|
|
|
|
|
Microarray |
single-cell developing |
High |
N/A |
Parallel gene expression |
no sensitivity |
Protein base |
|
|
|
|
|
Protein array |
single-cell developing |
High |
N/A |
Parallel protein expression |
no sensitivity |
|
|