Figure 5. Schematic representation of TAIL PCR. This strategy involves three consecutive rounds of PCR, performed with a set of three nested T-DNA specific primers (SP₁, SP₂ and SP₃) and a small, arbitrary primer. The positions of three nested primers in the T-DNA region are indicated by blue, red and green arrows respectively. AD is arbitrary degenerate primer indicated by purple arrow. T-DNA flanking region is indicated by green line.