Fig 7. Schematic representation of inverse PCR. This involves restriction digestion of genomic DNA from mutant plant with an appropriate enzyme that cuts (preferably cuts once within the T-DNA) followed by self-ligation. The circularized ligation products are used for PCR amplification using appropriate primers from the T-DNA region. The flanking plant DNA is represented by green line. The appropriate primers (forward and reverse) are indicated by blue and red arrows.